In this animated series, Peter Parker is a brilliant but insecure teenager who is bitten by a radioactive spider and gains superpowers. He becomes the superhero Spider-Man and must balance his new responsibilities with his daily life as a high school student. Peter must confront various villains, including Doctor Octopus, Venom, and the Vulture, while also navigating his relationships with his friends and family. As Spider-Man, Peter must learn to use his powers wisely and protect the city from danger. The series features a talented voice cast, including Robbie Daymond as Peter Parker/Spider-Man and Laura Bailey as Gwen Stacy/Ghost-Spider.

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Some TV shows that have similar genres and were produced around the same time as Marvel's Spider-Man include Pokémon Master Journeys, Masters of the Universe: Revolution, Baymax!, My Adventures with Superman, Hilda, Wow! Wow! Wubbzy!.